Detailed view for LmjF.18.1480

Basic information

TDR Targets ID: 25923
Leishmania major, NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5228 | Length (AA): 258 | MW (Da): 28684 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10588   NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
PF13510   2Fe-2S iron-sulfur cluster binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008137   NADH dehydrogenase (ubiquinone) activity  
GO:0016020   membrane  
GO:0009055   electron carrier activity  
GO:0051536   iron-sulfur cluster binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0042773   ATP synthesis coupled electron transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
34 249 2fug (3) 1 248 33.00 0 1 1.09 0.25
34 111 1awd () 2 90 19.00 0.000073 0.63 0.31 -0.77
37 194 1rm6 (C) 7 151 18.00 0 0.35 0.660303 -0.02
37 245 3c8y (A) 4 214 17.00 0 1 1.04598 -0.04
39 236 2fug (3) 6 235 36.00 0 1 1.13494 0.05
47 110 3b2g (A) 20 93 23.00 0 0.73 0.173962 -0.22

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128008)

Species Accession Gene Product
Arabidopsis thaliana AT5G37510   NADH-ubiquinone oxidoreductase subunit
Brugia malayi Bm1_52130   NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor
Candida albicans CaO19.7590   frameshifted
Candida albicans CaO19.7591   frameshifted
Caenorhabditis elegans CELE_Y45G12B.1   Protein NUO-5, isoform B
Dictyostelium discoideum DidioMp37   ORF688
Drosophila melanogaster Dmel_CG2286   NADH:ubiquinone reductase 75kD subunit precursor
Escherichia coli b2283   NADH:ubiquinone oxidoreductase, chain G
Echinococcus granulosus EgrG_001075900   NADH dehydrogenase ubiquinone Fe S protein 1
Echinococcus multilocularis EmuJ_001075900   NADH dehydrogenase (ubiquinone) Fe S protein 1
Homo sapiens ENSG00000023228   NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Leishmania braziliensis LbrM.18.1520   NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
Leishmania donovani LdBPK_181460.1   NADH-ubiquinone oxidoreductase complex I subunit, putative
Leishmania infantum LinJ.18.1460   NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
Leishmania major LmjF.18.1480   NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
Leishmania mexicana LmxM.18.1480   NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
Loa Loa (eye worm) LOAG_05732   hypothetical protein
Loa Loa (eye worm) LOAG_08633   NADH-ubiquinone oxidoreductase 75 kDa subunit
Mus musculus ENSMUSG00000025968   NADH dehydrogenase (ubiquinone) Fe-S protein 1
Mycobacterium tuberculosis Rv3151   Probable NADH dehydrogenase I (chain G) NuoG (NADH-ubiquinone oxidoreductase chain G)
Mycobacterium ulcerans MUL_2465   NADH dehydrogenase subunit G
Oryza sativa 4333901   Os03g0713400
Schistosoma japonicum Sjp_0100330   IPR015405,NADH-quinone oxidoreductase, chain G, C-terminal,domain-containing
Schistosoma japonicum Sjp_0204840   ko:K03934 NADH dehydrogenase (ubiquinone) Fe-S protein 1, putative
Schistosoma mansoni Smp_038870.2   NADH-ubiquinone oxidoreductase
Schistosoma mansoni Smp_038870.1   NADH-ubiquinone oxidoreductase
Schmidtea mediterranea mk4.070622.00   NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Schmidtea mediterranea mk4.000863.07   NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Schmidtea mediterranea mk4.000863.06   NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Schmidtea mediterranea mk4.000863.09  
Trypanosoma brucei gambiense Tbg972.10.15100   electron transfer protein, putative,ferredoxin, putative
Trypanosoma brucei Tb927.10.12540   NADH-ubiquinone oxidoreductase complex I subunit, putative
Trypanosoma congolense TcIL3000_10_11160   NADH-ubiquinone oxidoreductase complex I subunit, putative
Trypanosoma cruzi TcCLB.511261.30   NADH-ubiquinone oxidoreductase complex I subunit, putative
Trypanosoma cruzi TcCLB.509809.10   NADH-ubiquinone oxidoreductase complex I subunit, putative
Wolbachia endosymbiont of Brugia malayi Wbm0376   NADH dehydrogenase subunit G

Essentiality

LmjF.18.1480 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12540 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.12540 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12540 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12540 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2283 Escherichia coli non-essential goodall
CELE_Y45G12B.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y45G12B.1 Caenorhabditis elegans larval arrest wormbase
CELE_Y45G12B.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.18.1480 (Leishmania major), NADH:ubiquinone oxidoreductase 78 Kd subunit- like protein
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